Coomassie Brilliant Blue R 250

Coomassie Brilliant Blue R 250 Specification

Ph Level
Aqueous solution pH 7 (approx.)

HS Code
3204.12.94

Purity
>85%

Other Names
C.I. 42660, Acid Blue 83, Brilliant Blue R

Structural Formula
[Structural formula available upon request]

Poisonous
Yes

Smell
Odorless

Form
Solid, crystalline powder

Solubility
Soluble in water, methanol, ethanol, and slightly soluble in acetic acid

Melting Point
>300C (decomposes)

Molecular Formula
C45H44N3NaO7S2

Density
1.5 Gram per cubic centimeter(g/cm3)

Molecular Weight
825.97 g/mol

Taste
Odorless, tasteless

Storage
Store at room temperature, dry, tightly sealed, protected from light

Boiling point
Not applicable (decomposes)

Shape
Powder, crystalline solid

Classification
Anionic triphenylmethane dye, Biological stain

Chemical Name
Coomassie Brilliant Blue R 250

CAS No
6104-59-2

EINECS No
239-290-0

Grade
Laboratory Grade, Analytical Grade

Standard
Meets laboratory/analytical standards

Type
Dye, Biological Stain

Usage
Used in protein staining, laboratory analysis

Main Material
Coomassie Brilliant Blue R-250 dye

Application
Biological stain, protein quantification, electrophoresis

Stability
Stable under recommended storage conditions

Maximum Absorbance ( max)
~ 595 nm in aqueous solution

Packaging Size
10g, 25g, 100g, customized on request

Shelf Life
5 years when stored properly

Hazard Statements
Not classified as hazardous under normal laboratory conditions

Color Index Number
CI 42660

Appearance
Dark blue to purple crystalline powder

Light Sensitivity
Sensitive to light, store protected from light

Coomassie Brilliant Blue R 250 Trade Information

Minimum Order Quantity
50 Kilograms

Payment Terms
Cash in Advance (CID)

Supply Ability
100 Kilograms Per Week

Delivery Time
1 Week

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Asia

Main Domestic Market
All India

About Coomassie Brilliant Blue R 250

Coomassie Brilliant Blue R-250 is a dye used in laboratories to stain and quantify proteins. It's a fast-acting, sensitive dye that can be used on standard gels, IEF gels, and SDS gels. Here are some other properties of Coomassie Brilliant Blue R-250:

Staining sensitivity: 50-100 ng/band
Appearance: Blue to dark brown powder
Shelf life: 5 years
Storage temperature: Room temperature
Purity: >70%
Hazmat: Non-hazardous

The "R" in the dye's name stands for "red" because the dye has a slight reddish tint. The "250" originally indicated the dye's purity. Coomassie Brilliant Blue R-250 is also used in the Bradford Assay to quantify the concentration of protein in a solution.

Sihauli Chemicals is one of the leading Coomassie Brilliant Blue R-250 Manufacturer and Exporter form India. Sihauli Chemicals Supply Coomassie Brilliant Blue R-250 to Asia, Africa, Europe, North & South America and Oceania.

Our Global Presense:

Asia: Bangladesh, Burma (Myanmar), China, Hong Kong (UK), Indonesia, Iran, Israel, Japan, Jordan, Kampuchea (Cambodia), Kuwait, Laos, Lebanon, Macao (Portuguese), Malaysia, Maldives, Mongolia, Nepal, Oman, Philippines, Qatar, Saudi Arabia, Singapore, South Korea, Sri Lanka, Taiwan, Thailand, Turkey, United Arab Emirates, Vietnam.

Europe: Albania, Andorra, Austria, Belgium, Bulgaria, Cyprus, Czechoslovakia, Denmark, Finland, France, German Democratic Republic, Gibraltar, Greece, Hungary, Iceland, Ireland, Italy, Liechtenstein, Luxembourg, Malta, Monaco, Netherlands, Norway, Poland, Portugal, Romania, San Marino, Spain, Sweden, Switzerland, United Kingdom, U.S.S.R., Yugoslavia. Africa: Algeria, Angola, Egypt, Ethiopia, Gabon, Ghana, Kenya, Liberia, Libya, Madagascar, Mauritius, Morocco, Mozambique, Namibia, Nigeria, Reunion (France), Seychelles, Somalia, South Africa, Sudan, Tanzania, Tunisia, Uganda, Zambia, Zimbabwe.

North America: Bahamas, Barbados, Bermuda, British Virgin Islands, Canada, Costa Rica, Cuba, Dominica, El Salvador, Greenland (Denmark), Grenada, Guatemala, Haiti, Honduras, Jamaica, Martinique, Mexico, Montserrat, Nicaragua, Panama, Puerto Rico (USA), St. Vincent & the Grenadines, Trinidad & Tobago, USA, Virgin Islands.

South America: Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, French Guiana, Guyana, Paraguay, Peru, Venezuela.

Oceania: Australia, New Zealand, Solomon Islands, Wallis & Futuna Islands (France).

Accurate Protein Quantification

Coomassie Brilliant Blue R 250 is relied upon for its ability to bind selectively to proteins, facilitating accurate measurements in studies that require precise quantification. Its high solubility and sharp absorbance at ~595 nm make it an excellent choice for colorimetric protein assays such as the Bradford method.

Versatile Laboratory Applications

With its strong staining properties, Coomassie Brilliant Blue R 250 is indispensable for protein visualization in electrophoresis gels. Its consistency and laboratory-grade purity ensure reliable results in both research and industrial laboratories, catering to protein analysis, diagnostics, and educational purposes alike.

FAQ's of Coomassie Brilliant Blue R 250:

Q: How should Coomassie Brilliant Blue R 250 be stored to maintain its stability?

A: To preserve its stability and shelf life, Coomassie Brilliant Blue R 250 should be stored at room temperature in a dry, tightly sealed container, protected from light exposure, as it is sensitive to light.

Q: What are the main uses of Coomassie Brilliant Blue R 250 in the laboratory?

A: It is primarily used as a biological stain for protein quantification assays and electrophoresis, making protein bands visible for analysis in biochemical and molecular biology research.

Q: When should I use Coomassie Brilliant Blue R 250 instead of other protein stains?

A: Coomassie Brilliant Blue R 250 is preferred when you need a quick, reliable, and well-established method for detecting total proteins in gels, especially using colorimetric techniques like the Bradford assay.

Q: Where is Coomassie Brilliant Blue R 250 most effectively applied in experimental procedures?

A: This dye is best utilized in laboratories for staining proteins separated by SDS-PAGE or similar gel electrophoresis techniques and for quantifying protein concentrations in solution.

Q: What are the safety considerations for handling Coomassie Brilliant Blue R 250?

A: It is not classified as hazardous or poisonous under normal laboratory conditions. Use standard laboratory safety precautions, and avoid ingestion or inhalation.

Q: How does Coomassie Brilliant Blue R 250 benefit protein analysis?

A: It provides sensitive and consistent staining, resulting in distinct protein bands that facilitate accurate quantification and reproducible results in protein research.

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