About Diethyl pyrocarbonate (DEPC) Manufacturers, Suppliers, Exporters From Vasai Mumbai
Diethyl pyrocarbonate (DEPC) is a colorless, viscous liquid that is also known as diethyl dicarbonate. It is a strong but incomplete RNase inhibitor. Here are some properties of DEPC:
- Appearance: Clear, colorless liquid
- Density: 1.1201.122 g/ml at 20C
- Refractive index: 1.3971.398 at 20C
- Molecular weight: 162.14 g/mol
- Boiling point: 93C (1013 hPa)
- Flash point: 69C
- Storage temperature: Refrigerator
DEPC is soluble in ethanol, ether, acetone, and hydrocarbons, and slowly dissolves in water. It has a half-life of approximately 30 minutes in water. DEPC can be used as a modification reagent for His and Tyr residues in proteins. It can also be used as a nuclease inhibitor and as a probe for structural disruptions in dsDNA. Once opened, a bottle of DEPC should not be kept for more than 9 months. Sihauli Chemicals is one of the leadingDiethyl pyrocarbonate (DEPC) Manufacturer and Exporter form India. Sihauli Chemicals Supply Diethyl pyrocarbonate (DEPC) to Asia, Africa, Europe, North & South America and Oceania.Our Global Presense:
Asia: Bangladesh, Burma (Myanmar), China, Hong Kong (UK), Indonesia, Iran, Israel, Japan, Jordan, Kampuchea (Cambodia), Kuwait, Laos, Lebanon, Macao (Portuguese), Malaysia, Maldives, Mongolia, Nepal, Oman, Philippines, Qatar, Saudi Arabia, Singapore, South Korea, Sri Lanka, Taiwan, Thailand, Turkey, United Arab Emirates, Vietnam.
Europe: Albania, Andorra, Austria, Belgium, Bulgaria, Cyprus, Czechoslovakia, Denmark, Finland, France, German Democratic Republic, Gibraltar, Greece, Hungary, Iceland, Ireland, Italy, Liechtenstein, Luxembourg, Malta, Monaco, Netherlands, Norway, Poland, Portugal, Romania, San Marino, Spain, Sweden, Switzerland, United Kingdom, U.S.S.R., Yugoslavia. Africa: Algeria, Angola, Egypt, Ethiopia, Gabon, Ghana, Kenya, Liberia, Libya, Madagascar, Mauritius, Morocco, Mozambique, Namibia, Nigeria, Reunion (France), Seychelles, Somalia, South Africa, Sudan, Tanzania, Tunisia, Uganda, Zambia, Zimbabwe.
North America: Bahamas, Barbados, Bermuda, British Virgin Islands, Canada, Costa Rica, Cuba, Dominica, El Salvador, Greenland (Denmark), Grenada, Guatemala, Haiti, Honduras, Jamaica, Martinique, Mexico, Montserrat, Nicaragua, Panama, Puerto Rico (USA), St. Vincent & the Grenadines, Trinidad & Tobago, USA, Virgin Islands.
South America: Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, French Guiana, Guyana, Paraguay, Peru, Venezuela.
Oceania: Australia, New Zealand, Solomon Islands, Wallis & Futuna Islands (France).Premium Quality Molecular Biology ReagentOur Diethyl pyrocarbonate is manufactured and supplied according to stringent quality controls to ensure exceptional purity and performance, specifically for molecular biology applications. As a global exporter and supplier based in Vasai, Mumbai, we deliver DEPC compliant with international standards, making it suitable for research labs worldwide. Packaged in secure bottles of various sizes, DEPC is offered with a two-year shelf life, provided it is stored properly to guarantee stability and effectiveness.
Safe Storage and Handling ProtocolsDEPC is classified under Hazard Class 6.1 as it is toxic and must be transported according to UN hazardous chemical regulations. It should be stored in cool, dry locations, protected from moisture and light. The compound is incompatible with strong acids, bases, and oxidizing agents. Always handle DEPC using appropriate protective equipment in well-ventilated environments, and follow local safety guidelines when disposing of unused material.
Versatile Applications in LaboratoriesDEPC is widely utilized in molecular biology for the inactivation of RNase enzymes, essential for preparing RNase-free reagents and equipment. Its solubility profile makes it ideal for treating laboratory water, buffers, and glassware. By ensuring the elimination of RNase contaminations, DEPC enhances the reliability and integrity of sensitive RNA-based assays and experiments.
FAQ's of Diethyl pyrocarbonate (DEPC) Manufacturers, Suppliers, Exporters From Vasai Mumbai:
Q: How should Diethyl pyrocarbonate be stored to maintain its stability and shelf life?
A: To maintain DEPC's stability and ensure a shelf life of up to two years, it should be stored in tightly sealed containers in a cool, dry place away from moisture and direct light. Avoid exposing DEPC to strong acids, bases, or oxidizing agents, and always store separately from incompatible substances.
Q: What is the primary use of DEPC in molecular biology laboratories?
A: DEPC is primarily used to inactivate RNase enzymes, which helps prevent RNA degradation during laboratory procedures. It is commonly applied to water, buffers, solutions, and laboratory equipment to create an RNase-free working environment essential for reliable RNA analyses.
Q: When is it safe to use DEPC-treated solutions, and what precautions should be followed?
A: DEPC-treated solutions should only be used after thorough treatment and autoclaving to neutralize residual DEPC, ensuring that RNA samples will not be chemically altered. Proper lab safety protocols-including the use of gloves and fume hoods-should be observed due to the compound's toxicity and potential environmental hazards.
Q: Where is DEPC manufactured and exported from, and what are the available packaging options?
A: We manufacture DEPC in Vasai, Mumbai, India, and export it globally across Asia, Europe, America, and Africa. Packaging options include secure 100 ml, 500 ml, and 1-litre bottles, catering to various laboratory needs and order sizes.
Q: What benefits does using molecular biology grade DEPC offer in research applications?
A: Using molecular biology grade DEPC provides high purity, ensuring the effective inactivation of RNases without introducing contaminants that could interfere with sensitive RNA experiments. This enhances the reliability, reproducibility, and accuracy of RNA-based research methods.
Q: How is DEPC transported, and what regulations apply due to its hazard classification?
A: As a toxic substance classified under Hazard Class 6.1, DEPC is transported in compliance with UN regulations for hazardous chemicals. Proper labeling, specialized containers, and strict adherence to international shipping standards ensure the compound's safe delivery.