Coomassie Brilliant Blue G-250 (CBBG)

Coomassie Brilliant Blue G-250 (CBBG) Specification

Form
Solid/Powder

Solubility
Soluble in water, ethanol, methanol

Purity
90%

Molecular Formula
C47H48N3NaO7S2

Storage
Store tightly closed at room temperature, away from light and moisture

Poisonous
Yes

Other Names
CBBG, Brilliant Blue G

Structural Formula
See structure image

Boiling point
Not applicable (decomposes before boiling)

Melting Point
>300C (decomposes)

Ph Level
~7 (in solution)

Smell
Odorless

Molecular Weight
854.02 g/mol

Shape
Powder

HS Code
3204.12

Classification
Triphenylmethane dye

Chemical Name
Coomassie Brilliant Blue G-250

CAS No
6104-58-1

EINECS No
239-289-5

Grade
Laboratory/Analytical Reagent Grade

Standard
Meets laboratory analytical standards

Type
Organic dye

Usage
Laboratory reagent, stain for proteins in gels

Main Material
Coomassie Brilliant Blue G-250 dye

Application
Protein staining, analytical chemistry, electrophoresis staining (Bradford protein assay)

Non Toxic
Yes

Transport Information
Non-hazardous for transport

Safety Precautions
Avoid inhalation, ingestion, and contact with skin and eyes; use gloves and safety glasses

Appearance
Dark blue to greenish-blue powder

Stability
Stable under recommended storage conditions

Expiry/Shelf life
2-3 years if stored properly

Sensitivity
Sensitive to light and moisture

Packing
Sealed vial/bottle, customizable on request

Absorption maximum (max)
595 nm (in water)

Coomassie Brilliant Blue G-250 (CBBG) Trade Information

Minimum Order Quantity
50 Kilograms

Payment Terms
Cash in Advance (CID)

Supply Ability
200 Kilograms Per Week

Delivery Time
2 Week

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Asia

Main Domestic Market
All India

About Coomassie Brilliant Blue G-250 (CBBG)

Coomassie Brilliant Blue G-250 (CBBG) is a triphenylmethane dye that is commonly used in analytical biochemistry. It is a member of the Coomassie family of dyes, which were originally developed for the textile industry.
CBBG is used in a variety of ways, including:

As an analytical dye in SDS-PAGE and BN-PAGE
In the Bradford Method to determine protein concentration
In SDS gels to stain proteins with minimal background color
In the dye-binding protein assay

CBBG differs from Coomassie brilliant blue R-250 by the addition of two methyl groups. CBBG binds to proteins through a combination of hydrophobic interactions and heteropolar bonding with basic amino acids.

Sihauli Chemicals is one of the leading
Coomassie Brilliant Blue G-250 (CBBG) Manufacturer and Exporter form India. Sihauli Chemicals Supply Coomassie Brilliant Blue G-250 (CBBG) to Asia, Africa, Europe, North & South America and Oceania.

Our Global Presense:

Asia: Bangladesh, Burma (Myanmar), China, Hong Kong (UK), Indonesia, Iran, Israel, Japan, Jordan, Kampuchea (Cambodia), Kuwait, Laos, Lebanon, Macao (Portuguese), Malaysia, Maldives, Mongolia, Nepal, Oman, Philippines, Qatar, Saudi Arabia, Singapore, South Korea, Sri Lanka, Taiwan, Thailand, Turkey, United Arab Emirates, Vietnam.

Europe: Albania, Andorra, Austria, Belgium, Bulgaria, Cyprus, Czechoslovakia, Denmark, Finland, France, German Democratic Republic, Gibraltar, Greece, Hungary, Iceland, Ireland, Italy, Liechtenstein, Luxembourg, Malta, Monaco, Netherlands, Norway, Poland, Portugal, Romania, San Marino, Spain, Sweden, Switzerland, United Kingdom, U.S.S.R., Yugoslavia. Africa: Algeria, Angola, Egypt, Ethiopia, Gabon, Ghana, Kenya, Liberia, Libya, Madagascar, Mauritius, Morocco, Mozambique, Namibia, Nigeria, Reunion (France), Seychelles, Somalia, South Africa, Sudan, Tanzania, Tunisia, Uganda, Zambia, Zimbabwe.

North America: Bahamas, Barbados, Bermuda, British Virgin Islands, Canada, Costa Rica, Cuba, Dominica, El Salvador, Greenland (Denmark), Grenada, Guatemala, Haiti, Honduras, Jamaica, Martinique, Mexico, Montserrat, Nicaragua, Panama, Puerto Rico (USA), St. Vincent & the Grenadines, Trinidad & Tobago, USA, Virgin Islands.

South America: Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, French Guiana, Guyana, Paraguay, Peru, Venezuela.

Oceania: Australia, New Zealand, Solomon Islands, Wallis & Futuna Islands (France).

Exceptional Sensitivity for Protein Analysis

Coomassie Brilliant Blue G-250 provides outstanding sensitivity for detecting and quantifying proteins, making it indispensable for electrophoresis staining and the Bradford assay. Its ability to bind selectively to proteins allows for reliable and reproducible results in research and diagnostic labs.

Stable and Reliable Laboratory Reagent

When stored properly-tightly closed, at room temperature, and shielded from light and moisture-this dye maintains its integrity and performance for up to 2-3 years. Its solid, odorless powdered form ensures easy measurement and storage, enhancing laboratory workflow.

FAQ's of Coomassie Brilliant Blue G-250 (CBBG):

Q: How should Coomassie Brilliant Blue G-250 be stored to maintain its stability?

A: Coomassie Brilliant Blue G-250 should be stored tightly closed at room temperature, away from light and moisture, to ensure stability and maintain its effectiveness for up to 2-3 years.

Q: What is the primary application of Coomassie Brilliant Blue G-250 in the laboratory?

A: CBBG is primarily used as a protein stain in analytical chemistry, notably for staining proteins in gels after electrophoresis and in the Bradford protein assay for quantitative protein estimation.

Q: When should Coomassie Brilliant Blue G-250 be replaced in the laboratory?

A: It is recommended to replace CBBG after its expiry period of 2-3 years, or if the powder appears degraded or has been exposed to light and moisture, which might compromise its staining properties.

Q: Where can Coomassie Brilliant Blue G-250 be used?

A: This dye is suitable for use in laboratories specializing in protein analysis, research, diagnostics, and high-precision analytical chemistry due to its reliable staining efficiency and laboratory-grade quality.

Q: What is the process for using Coomassie Brilliant Blue G-250 in protein staining?

A: To stain proteins, dissolve the powder in water, ethanol, or methanol as required, apply to the gel post-electrophoresis, and monitor the absorption at 595 nm for quantitative assays, such as the Bradford protein assay.

Q: What safety precautions should be taken when handling Coomassie Brilliant Blue G-250?

A: Always use gloves and safety glasses when handling CBBG. Avoid inhalation, ingestion, and skin or eye contact as the dye is poisonous; proper personal protective equipment (PPE) is necessary.

Q: What are the benefits of using Coomassie Brilliant Blue G-250 in analytical applications?

A: CBBG offers rapid, sensitive, and specific protein detection, is non-hazardous for transport, and provides reproducible results, making it a valuable reagent in research and biochemical analysis.

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